Purpose of Hemolysis Testing for Sterile Media Bottles

In modern cell culture technology, sterile media bottles are commonly used containers that hold various essential solutions for cell culture, such as media, sera, and buffers.

In modern cell culture technology, sterile media bottles are commonly used containers that hold various essential solutions for cell culture, such as media, sera, and buffers. To ensure stable culture conditions and healthy cell growth, sterile media bottles must undergo rigorous hemolysis testing to evaluate their tolerance and safety when in contact with red blood cells.

Hemolysis refers to the process where the cell membrane is damaged or destroyed, causing the contents of the cell (such as hemoglobin) to leak into the surrounding environment. This phenomenon can be triggered by various factors, including changes in osmotic pressure, chemical toxins, and mechanical forces. In the cell culture process, if the sterile media bottles used have hemolysis issues, it could affect the composition and pH of the culture media, thereby impacting cell health and the accuracy of experimental results.

To ensure that the quality of sterile media bottles meets the required standards, hemolysis testing primarily involves using fresh anticoagulated rabbit blood as positive and negative controls, exposing the test samples to it, and then measuring the absorbance at a specific wavelength (such as 545 nm) with a UV-Visible spectrophotometer to evaluate the hemolysis rate.

Through rigorous hemolysis testing, manufacturers of serum culture bottles can ensure that their products meet high pharmaceutical standards and experimental requirements, guaranteeing the reliability of cell culture and the accuracy of results. This process is not only a crucial part of quality control but also a key step in ensuring the safety of cell research and biopharmaceutical applications. It reflects the continuous effort to ensure the quality and reliability of cell culture in medical research and clinical applications.